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srlB:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

srlB

Mnemonic

Sorbitol

Synonyms

ECK2699, b2704, JW2673, gutB[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

60.9 minutes 

MG1655: 2825384..2825755
gbrowse_img.php?coord=2825384..2825755&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2722221..2722592
gbrowse_img.php?coord=2722221..2722592&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 2826018..2826389
gbrowse_img.php?coord=2826018..2826389&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2917926..2918297
gbrowse_img.php?coord=2917926..2918297&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 2711196..2711567
gbrowse_img.php?coord=2711196..2711567&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔsrlB (Keio:JW2673)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10108[3]

srlBI17T

I17T

(in strain: ECOR 6, ECOR 28, ECOR 35, ECOR 37, ECOR 51, ECOR 58, ECOR 61, ECOR 66 and ECOR 69)

Strain variation; seeded from UniProt:P05706

srlBA51E

A51E

(in strain: ECOR 58)

Strain variation; seeded from UniProt:P05706

srlBS59T

S59T

(in strain: ECOR 28, ECOR 37, ECOR 58 and ECOR 69)

Strain variation; seeded from UniProt:P05706

srlBQ62R

Q62R

(in strain: ECOR 51)

Strain variation; seeded from UniProt:P05706

srlBN92S

N92S

(in strain: ECOR 51, ECOR 61 and ECOR 66)

Strain variation; seeded from UniProt:P05706

ΔsrlB779::kan

PMID:16738554[2]

CGSC:103012


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW2673

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCACCGTTATTTATCAGACCAC

Primer 2:CCtTCCTTAACAGATTCAAACTT

pheA18::Tn10

Linked marker

CAG12158 = CGSC7421[3]

est. P1 cotransduction: % [5]

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[3]

est. P1 cotransduction: 99% [5]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10970

EchoBASE (EcoliWiki Page)

EchoBASE:EB0963

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2704

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3258

EcoGene (EcoliWiki Page)

EcoGene:EG10970

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:SRLB

CGSC (EcoliWiki Page)

CGSC:11886

ASAP (EcoliWikiPage)

ASAP:ABE-0008894


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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