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srlE:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

srlE

Mnemonic

Sorbitol

Synonyms

ECK2698, b2703, JW5430, gutE, srlA_2, gutA, sbl, srlA-2[1][2]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

60.88 minutes 

MG1655: 2824414..2825373
gbrowse_img.php?coord=2824414..2825373&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2721251..2722210
gbrowse_img.php?coord=2721251..2722210&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 2825048..2826007
gbrowse_img.php?coord=2825048..2826007&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2916956..2917915
gbrowse_img.php?coord=2916956..2917915&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 2710226..2711185
gbrowse_img.php?coord=2710226..2711185&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔsrlE (Keio:JW5430)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC11386[4]

srlE::Tn5KAN

PMID:12519969[5]

ASAP

At position 722 in Plus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20888

srlE::Tn5KAN

PMID:12519969[5]

ASAP

At position 722 in Plus orientation, contains plasmid pKD46.
ASAP strain name: FB20889

ΔsrlE778::kan

PMID:16738554[3]

CGSC:103016


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW5430

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCACGCATATTCGGATCGAAAA

Primer 2:CCTTGATAGATAAAACCGGAGAC

pheA18::Tn10

Linked marker

CAG12158 = CGSC7421[4]

est. P1 cotransduction: % [7]

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[4]

est. P1 cotransduction: 96% [7]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10969

EchoBASE (EcoliWiki Page)

EchoBASE:EB4116

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2703

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3257

EcoGene (EcoliWiki Page)

EcoGene:EG10969

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:SRLE_2

CGSC (EcoliWiki Page)

CGSC:155

ASAP (EcoliWikiPage)

ASAP:ABE-0008892


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 Glasner JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res 31: 147-51 PubMed EcoliWiki page
  6. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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