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surA:On One Page

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Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

surA

Gene Synonym(s)

ECK0054, b0053, JW0052[1]

Product Desc.

peptidyl-prolyl cis-trans isomerase (PPIase)[2][3]

Periplasmic OM porin chaperone, has PPIase activity; required for stationary-phase survival[4]

Product Synonyms(s)

peptidyl-prolyl cis-trans isomerase (PPIase)[1], B0053[2][1], SurA[2][1]

Function from GO

Molecular Function

Biological Process

Cellular Component

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): imp-surA-pdxA[2], surA-pdxA-rsmA-apaGH[2], surA-pdxA-ksgA-apaGH

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(surA)    Textpresso(surA)

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Notes

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Parvulin (ppiC) family. SurA contains two PPIase domains. PPIase domain I is inactive. PPIase activity is not required for porin maturation. An fkpA, surA, ppiB, ppiD quadruple mutant is viable.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

surA

Mnemonic

Survival

Synonyms

ECK0054, b0053, JW0052[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

1.15 minutes 

MG1655: 54702..53416
gbrowse_img.php?coord=53416..54702&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 58897..57611
gbrowse_img.php?coord=57611..58897&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 54702..53416
gbrowse_img.php?coord=53416..54702&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 54702..53416
gbrowse_img.php?coord=53416..54702&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 53416..54702
gbrowse_img.php?coord=53416..54702&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔsurA (Keio:JW0052)

deletion

deletion

PMID:16738554

Shigen
CGSC8362[5]

ΔsurA765::kan

PMID:16738554

CGSC:103072


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0052

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAGAACTGGAAAACGCTGCT

Primer 2:CCGTTGCTCAGGATTTTAACGTA

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[5]

est. P1 cotransduction: 46% [6]
Synonyms:car-96::Tn10

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[5]

est. P1 cotransduction: 31% [6]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10985

EchoBASE (EcoliWiki Page)

EchoBASE:EB0978

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0053

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0066

EcoGene (EcoliWiki Page)

EcoGene:EG10985

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:SURA

CGSC (EcoliWiki Page)

CGSC:30326

ASAP (EcoliWikiPage)

ASAP:ABE-0000180


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

SurA

Synonyms

peptidyl-prolyl cis-trans isomerase (PPIase)[1], B0053[2][1], SurA[2][1]

Product description

peptidyl-prolyl cis-trans isomerase (PPIase)[2][3]

Periplasmic OM porin chaperone, has PPIase activity; required for stationary-phase survival[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0051082

unfolded protein binding

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01183

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0042597

periplasmic space

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0574

C

Seeded from EcoCyc 12.5 [7]

complete

GO:0016853

isomerase activity

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR000297
SP_KW:KW-0413

F

Seeded from EcoCyc [8]

complete

GO:0006457

protein folding

GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

SP_KW:KW-0697
HAMAP:MF_01183

P

Seeded from EcoCyc 12.5 [7]

complete

GO:0003755

peptidyl-prolyl cis-trans isomerase activity

GO_REF:0000003
GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

EC:5.2.1.8
SP_KW:KW-0697
HAMAP:MF_01183

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0030288

outer membrane-bounded periplasmic space

C

Seeded from Riley et al 2006 [1].

required fields missing

GO:0005515

protein binding

PMID:11226178
PMID:17908933
PMID:18165306

IPI: Inferred from Physical Interaction

UniProtKB:P02932
UniProtKB:P0A940
UniProtKB:P0A940

F

Seeded from EcoCyc [8]

complete

GO:0042277

peptide binding

PMID:11546789
PMID:14506253
PMID:15840585
PMID:17825319
PMID:17894549

IDA: Inferred from Direct Assay





F

Seeded from EcoCyc [8]

complete

GO:0042710

biofilm formation

PMID:18180259

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc [8]

complete

GO:0043165

Gram-negative-bacterium-type cell outer membrane assembly

PMID:17908933

IGI: Inferred from Genetic Interaction

UniProtKB:P0AEU7

P

Seeded from EcoCyc [8]

complete

GO:0050821

protein stabilization

PMID:11226178
PMID:17071751

IMP: Inferred from Mutant Phenotype


P

Seeded from EcoCyc [8]

complete

GO:0051085

chaperone cofactor-dependent protein folding

PMID:17071751

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc [8]

complete

GO:0009279

cell outer membrane

C

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0060274

maintenance of stationary phase

PMID:2165476

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006457

protein folding

PMID:8626309

IMP: Inferred from Mutant Phenotype

P

complete

GO:0050821

protein stabilization

PMID:8626309

IMP: Inferred from Mutant Phenotype

P

complete

GO:0003755

peptidyl-prolyl cis-trans isomerase activity

PMID:8985185

IDA: Inferred from Direct Assay

F

complete

GO:0043165

Gram-negative-bacterium-type cell outer membrane assembly

PMID:8985185

IMP: Inferred from Mutant Phenotype

P

complete

GO:0030288

outer membrane-bounded periplasmic space

PMID:8985185

IDA: Inferred from Direct Assay

C

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

dnaK

PMID:16606699

Experiment(s):EBI-1135459

Protein

hflB

PMID:16606699

Experiment(s):EBI-1135459

Protein

chbC

PMID:15690043

Experiment(s):EBI-891312

Protein

ilvA

PMID:15690043

Experiment(s):EBI-891312

Protein

lpdA

PMID:15690043

Experiment(s):EBI-891312

Protein

rplL

PMID:15690043

Experiment(s):EBI-891312

Protein

rpsN

PMID:15690043

Experiment(s):EBI-891312

Protein

ydaY

PMID:15690043

Experiment(s):EBI-891312

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

periplasm

Periplasm

PMID:9298646

EchoLocation:surA


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MKNWKTLLLG IAMIANTSFA APQVVDKVAA VVNNGVVLES DVDGLMQSVK LNAAQARQQL
PDDATLRHQI MERLIMDQII LQMGQKMGVK ISDEQLDQAI ANIAKQNNMT LDQMRSRLAY
DGLNYNTYRN QIRKEMIISE VRNNEVRRRI TILPQEVESL AQQVGNQNDA STELNLSHIL
IPLPENPTSD QVNEAESQAR AIVDQARNGA DFGKLAIAHS ADQQALNGGQ MGWGRIQELP
GIFAQALSTA KKGDIVGPIR SGVGFHILKV NDLRGESKNI SVTEVHARHI LLKPSPIMTD
EQARVKLEQI AADIKSGKTT FAAAAKEFSQ DPGSANQGGD LGWATPDIFD PAFRDALTRL
NKGQMSAPVH SSFGWHLIEL LDTRNVDKTD AAQKDRAYRM LMNRKFSEEA ASWMQEQRAS
AYVKILSN
Length

428

Mol. Wt

47.284 kDa

pI

7.0 (calculated)

Extinction coefficient

34,950 (calc based on 5 Y, 5 W, and C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences

motif

1-20

UniProt Manual:Signal Peptides

UniProt:P0ABZ6


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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10985-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0ABZ6

PFAM (EcoliWiki Page)

PFAM:PF00639

RefSeq (EcoliWiki Page)

RefSeq:NP_414595


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

lptD-surA-pdxA

surA-pdxA-rsmA-apaGH

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=54682..54722&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein+flip&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b0053 (EcoliWiki Page)

NCBI GEO profiles for surA

microarray

GenExpDB:b0053 (EcoliWiki Page)

Summary of data for surA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to surA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10985

EchoBASE (EcoliWiki Page)

EchoBASE:EB0978

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0053

EcoGene (EcoliWiki Page)

EcoGene:EG10985


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Dictyostelium discoideum

  • DDB0001410 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

SURA

From SHIGELLACYC

E. coli O157

SURA

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00639


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  8. ↑ 8.0 8.1 8.2 8.3 8.4 8.5 8.6 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70

Categories

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