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thiP:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

thiP

Mnemonic

Thiamin (and thiazole)

Synonyms

ECK0068, b0067, JW0066, sfuB, yabK[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

1.57 minutes 

MG1655: 74521..72911
gbrowse_img.php?coord=72911..74521&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 77325..75715
gbrowse_img.php?coord=75715..77325&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 74521..72911
gbrowse_img.php?coord=72911..74521&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

BW2952

NC_012759: 72910..74520
gbrowse_img.php?coord=72910..74520&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

yabK::Tn5KAN

PMID:12519969[2]

ASAP

At position 1064 in Plus orientation, contains plasmid pKD46.
ASAP strain name: FB20007

yabK::Tn5KAN

PMID:12519969[2]

ASAP

At position 1064 in Plus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20008

ΔthiP (Keio:JW0066)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8368[4]

ΔthiP774::kan

PMID:16738554[3]

CGSC:103144

Strain BW2952

T18C

C64T

A91G

T205G G211A C352G T268C C283G T298C G304A C344T T409C G418A A421G A503G C535A G802T A834C G843A T880C G893A G907T C943T C958G C1009T TA1074-1075CG T1099C C1005T G1117A C1159T C1171T A1174G G1177C G1295A A1300G C1312T A1324G T1398C T1411C

PMID:19376874[5]

Relative to MG1655.

Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0066

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGCAACGCGCCGTCAGCCGTT

Primer 2:CCGTCAGTTTTAACATTTCGCCC

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[4]

est. P1 cotransduction: 18% [7]
Synonyms:car-96::Tn10

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[4]

est. P1 cotransduction: 69% [7]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11573

EchoBASE (EcoliWiki Page)

EchoBASE:EB1533

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0067

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0083

EcoGene (EcoliWiki Page)

EcoGene:EG11573

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YABK

ASAP (EcoliWikiPage)

ASAP:ABE-0000246


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Glasner JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res 31: 147-51 PubMed EcoliWiki page
  3. 3.0 3.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Ferenci T et al. (2009) Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12. J Bacteriol 191: 4025-9 PubMed EcoliWiki page
  6. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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