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thiP:On One Page

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Standard Name

thiP

Gene Synonym(s)

ECK0068, b0067, JW0066, sfuB, yabK[1]

Product Desc.

SfuB[2][3];

Component of thiamin ABC transporter[2][3]

Thiamine and thiamine pyrophosphate ABC transporter[4]

Product Synonyms(s)

fused thiamin transporter subunits of ABC superfamily: membrane components[1], B0067[2][1], YabK[2][1], SfuB[2][1], ThiP[2][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): thiQ[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(thiP)    Textpresso(thiP)

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Notes

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Primarily characterized in Salmonella[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

thiP

Mnemonic

Thiamin (and thiazole)

Synonyms

ECK0068, b0067, JW0066, sfuB, yabK[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

1.57 minutes 

MG1655: 74521..72911
gbrowse_img.php?coord=72911..74521&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 77325..75715
gbrowse_img.php?coord=75715..77325&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 74521..72911
gbrowse_img.php?coord=72911..74521&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

BW2952

NC_012759: 72910..74520
gbrowse_img.php?coord=72910..74520&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

yabK::Tn5KAN

PMID:12519969

ASAP

At position 1064 in Plus orientation, contains plasmid pKD46.
ASAP strain name: FB20007

yabK::Tn5KAN

PMID:12519969

ASAP

At position 1064 in Plus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20008

ΔthiP (Keio:JW0066)

deletion

deletion

PMID:16738554

Shigen
CGSC8368[5]

ΔthiP774::kan

PMID:16738554

CGSC:103144

Strain BW2952

T18C

C64T

A91G

T205G G211A C352G T268C C283G T298C G304A C344T T409C G418A A421G A503G C535A G802T A834C G843A T880C G893A G907T C943T C958G C1009T TA1074-1075CG T1099C C1005T G1117A C1159T C1171T A1174G G1177C G1295A A1300G C1312T A1324G T1398C T1411C

PMID:19376874

Relative to MG1655.

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0066

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGCAACGCGCCGTCAGCCGTT

Primer 2:CCGTCAGTTTTAACATTTCGCCC

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[5]

est. P1 cotransduction: 18% [6]
Synonyms:car-96::Tn10

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[5]

est. P1 cotransduction: 69% [6]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11573

EchoBASE (EcoliWiki Page)

EchoBASE:EB1533

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0067

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0083

EcoGene (EcoliWiki Page)

EcoGene:EG11573

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YABK

ASAP (EcoliWikiPage)

ASAP:ABE-0000246


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ThiP

Synonyms

fused thiamin transporter subunits of ABC superfamily: membrane components[1], B0067[2][1], YabK[2][1], SfuB[2][1], ThiP[2][1]

Product description

SfuB[2][3];

Component of thiamin ABC transporter[2][3]

Thiamine and thiamine pyrophosphate ABC transporter[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0016021

integral to membrane

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR005947
SP_KW:KW-0812

C

Seeded from EcoCyc [7]

complete

GO:0016020

membrane

GO_REF:0000002
GO_REF:0000004
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR000515
SP_KW:KW-0397
SP_KW:KW-0472

C

Seeded from EcoCyc 12.5 [8]

complete

GO:0009276

Gram-negative-bacterium-type cell wall

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005947

C

Seeded from EcoCyc [7]

complete

GO:0006810

transport

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR000515
InterPro:IPR005947
SP_KW:KW-0813

P

Seeded from EcoCyc [7]

complete

GO:0005215

transporter activity

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000515
InterPro:IPR005947

F

Seeded from EcoCyc [7]

complete

GO:0009228

thiamin biosynthetic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0005886

plasma membrane

GO_REF:0000004
GO_REF:0000004
GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997
SP_KW:KW-1003
SP_SL:SL-0037

C

Seeded from EcoCyc [7]

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of thiamin ABC transporter

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MATRRQPLIP GWLIPGVSAT TLVVAVALAA FLALWWNAPQ DDWVAVWQDS YLWHVVRFSF
WQAFLSALLS VIPAIFLARA LYRRRFPGRL ALLRLCAMTL ILPVLVAVFG ILSVYGRQGW
LATLCQSLGL EWTFSPYGLQ GILLAHVFFN LPMASRLLLQ ALENIPGEQR QLAAQLGMRS
WHFFRFVEWP WLRRQIPPVA ALIFMLCFAS FATVLSLGGG PQATTIELAI YQALSYDYDP
ARAAMLALLQ MVCCLGLVLL SQRLSKAIAP GTTLLQGWRD PDDRLHSRIC DTVLIVLALL
LLLPPLLAVI VDGVNRQLPE VLAQPVLWQA LWTSLRIALA AGVLCVVLTM MLLWSSRELR
ARQKMLAGQV LEMSGMLILA MPGIVLATGF FLLLNNTIGL PQSADGIVIF TNALMAIPYA
LKVLENPMRD ITARYSMLCQ SLGIEGWSRL KVVELRALKR PLAQALAFAC VLSIGDFGVV
ALFGNDDFRT LPFYLYQQIG SYRSQDGAVT ALILLLLCFL LFTVIEKLPG RNVKTD
Length

536

Mol. Wt

59.535 kDa

pI

9.5 (calculated)

Extinction coefficient

111,380 - 112,630 (calc based on 12 Y, 17 W, and 10 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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tmhmm.php?gene=thiP&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:SFUB-MONOMER

UniProt (EcoliWiki Page)

UniProt:P31549

PFAM (EcoliWiki Page)

PFAM:PF00528

RefSeq (EcoliWiki Page)

RefSeq:NP_414609


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

thiQ

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=74501..74541&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein+flip&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b0067 (EcoliWiki Page)

NCBI GEO profiles for thiP

microarray

GenExpDB:b0067 (EcoliWiki Page)

Summary of data for thiP from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to thiP Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11573

EchoBASE (EcoliWiki Page)

EchoBASE:EB1533

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0067

EcoGene (EcoliWiki Page)

EcoGene:EG11573


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Anopheles gambiae

  • ENSANGP00000002232 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

YABK

From SHIGELLACYC

E. coli O157

YABK

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00528


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 7.4 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

Categories

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