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thiQ:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

thiQ

Mnemonic

Thiamin (and thiazole)

Synonyms

ECK0067, b0066, JW0065, sfuC, yabJ[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

1.56 minutes 

MG1655: 72927..72229
gbrowse_img.php?coord=72229..72927&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 75033..75731
gbrowse_img.php?coord=75033..75731&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 72927..72229
gbrowse_img.php?coord=72229..72927&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

BW2952

NC_012759: 72228..72926
gbrowse_img.php?coord=72228..72926&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

yabJ::Tn5KAN

PMID:12519969[2]

ASAP

At position 419 in Minus orientation, contains plasmid pKD46.
ASAP strain name: FB20006

ΔthiQ (Keio:JW0065)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8367[4]

ΔthiQ773::kan

PMID:16738554[3]

CGSC:103146


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0065

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCTTAAAACTGACTGATATCAC

Primer 2:CCACCCGTAATCCCCAATAGTG

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[4]

est. P1 cotransduction: 19% [6]
Synonyms:car-96::Tn10

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[4]

est. P1 cotransduction: 68% [6]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11572

EchoBASE (EcoliWiki Page)

EchoBASE:EB1532

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0066

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0082

EcoGene (EcoliWiki Page)

EcoGene:EG11572

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YABJ

ASAP (EcoliWikiPage)

ASAP:ABE-0000244


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Glasner JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res 31: 147-51 PubMed EcoliWiki page
  3. 3.0 3.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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