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thrA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ThrA

Synonyms

fused aspartokinase I[1], homoserine dehydrogenase I[1], B0002[2][1], ThrD[2][1], ThrA[2][1]

Product description

ThrA[2][3];

Component of aspartate kinase I[2]

Aspartokinase I and homoserine dehydrogenase I, bifunctional[4]

EC number (for enzymes)

Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus
GO:0016740

transferase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc [5]

complete

GO:0016597

amino acid binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002912

F

Seeded from EcoCyc [5]

complete

GO:0016491

oxidoreductase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0016301

kinase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0418

F

Seeded from EcoCyc [5]

complete

GO:0009088

threonine biosynthetic process

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0791

P

Seeded from EcoCyc [5]

complete

GO:0004412

homoserine dehydrogenase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000003

IEA: Inferred from Electronic Annotation

InterPro:IPR001342
InterPro:IPR011147
EC:1.1.1.3

F

Seeded from EcoCyc [5]

complete

GO:0004072

aspartate kinase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000003

IEA: Inferred from Electronic Annotation

InterPro:IPR001341
InterPro:IPR011147
EC:2.7.2.4

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0003824

catalytic activity

GO_REF:0000004
GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0021
SP_KW:KW-0511

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0005737

cytoplasm

C

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0005488

binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc [5]

complete

GO:0008152

metabolic process

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002912
InterPro:IPR016040

P

Seeded from EcoCyc [5]

complete

GO:0008652

amino acid biosynthetic process

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001048
InterPro:IPR001341
InterPro:IPR001342
InterPro:IPR018042
SP_KW:KW-0028

P

Seeded from EcoCyc [5]

complete

GO:0009067

aspartate family amino acid biosynthetic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011147

P

Seeded from EcoCyc [5]

complete

GO:0050661

NADP or NADPH binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005106

F

Seeded from EcoCyc [5]

complete

GO:0055114

oxidation reduction

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc [5]

complete

GO:0009086

methionine biosynthetic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0009090

homoserine_biosynthesis

PMID:11368768[7]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 12.5 [6]

GO:0009089

lysine_biosynthesis_via_diaminopimelate

PMID:11368768[7]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 12.5 [6]


Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of aspartate kinase I

could be indirect

Protein

glpD

PMID:16606699[8]

Experiment(s):EBI-1135292

Protein

zwf

PMID:16606699[8]

Experiment(s):EBI-1135292

Protein

ahpF

PMID:16606699[8]

Experiment(s):EBI-1135292

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

Cytoplasm

PMID:4562989[9], PMID:354697[10], PMID:7003595[11], PMID:6777249[12]

EchoLocation:thrA


Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc

MRVLKFGGTS VANAERFLRV ADILESNARQ GQVATVLSAP AKITNHLVAM IEKTISGQDA
LPNISDAERI FAELLTGLAA AQPGFPLAQL KTFVDQEFAQ IKHVLHGISL LGQCPDSINA
ALICRGEKMS IAIMAGVLEA RGHNVTVIDP VEKLLAVGHY LESTVDIAES TRRIAASRIP
ADHMVLMAGF TAGNEKGELV VLGRNGSDYS AAVLAACLRA DCCEIWTDVD GVYTCDPRQV
PDARLLKSMS YQEAMELSYF GAKVLHPRTI TPIAQFQIPC LIKNTGNPQA PGTLIGASRD
EDELPVKGIS NLNNMAMFSV SGPGMKGMVG MAARVFAAMS RARISVVLIT QSSSEYSISF
CVPQSDCVRA ERAMQEEFYL ELKEGLLEPL AVTERLAIIS VVGDGMRTLR GISAKFFAAL
ARANINIVAI AQGSSERSIS VVVNNDDATT GVRVTHQMLF NTDQVIEVFV IGVGGVGGAL
LEQLKRQQSW LKNKHIDLRV CGVANSKALL TNVHGLNLEN WQEELAQAKE PFNLGRLIRL
VKEYHLLNPV IVDCTSSQAV ADQYADFLRE GFHVVTPNKK ANTSSMDYYH QLRYAAEKSR
RKFLYDTNVG AGLPVIENLQ NLLNAGDELM KFSGILSGSL SYIFGKLDEG MSFSEATTLA
REMGYTEPDP RDDLSGMDVA RKLLILARET GRELELADIE IEPVLPAEFN AEGDVAAFMA
NLSQLDDLFA ARVAKARDEG KVLRYVGNID EDGVCRVKIA EVDGNDPLFK VKNGENALAF
YSHYYQPLPL VLRGYGAGND VTAAGVFADL LRTLSWKLGV
Length

820

Mol. Wt

89.121 kDa

pI

5.5 (calculated)

Extinction coefficient

51,800 - 53,300 (calc based on 20 Y, 4 W, and 12 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences


Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:ASPKINIHOMOSERDEHYDROGI-MONOMER

UniProt (EcoliWiki Page)

UniProt:P00561

PFAM (EcoliWiki Page)

PFAM:PF00696

RefSeq (EcoliWiki Page)

RefSeq:NP_414543


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  6. 6.0 6.1 6.2 6.3 6.4 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  7. 7.0 7.1 Chassagnole C et al. (2001) An integrated study of threonine-pathway enzyme kinetics in Escherichia coli. Biochem J 356: 415-23 PubMed EcoliWiki page
  8. 8.0 8.1 8.2 Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
  9. Falcoz-Kelly F et al. (1972) Revised structure of aspartokinase I-homoserine dehydrogenase I of Escherichia coli K12. Evidence for four identical subunits. Eur J Biochem 28: 507-19 PubMed EcoliWiki page
  10. Cossart-Gheerbrant P et al. (1978) The primary structure of Escherichia coli K12 aspartokinase I-homoserine dehydrogenase I-Isolation and characterisation of the peptides produced by cyanogen bromide. Biochim Biophys Acta 535: 206-15 PubMed EcoliWiki page
  11. Katinka M et al. (1980) Nucleotide sequence of the thrA gene of Escherichia coli. Proc Natl Acad Sci U S A 77: 5730-3 PubMed EcoliWiki page
  12. Kozlov IuI et al. (1980) [Cloning of threonine operon genes in Escherichia coli cells] Genetika 16: 66-77 PubMed EcoliWiki page

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