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thrA:On One Page

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Standard Name

thrA

Gene Synonym(s)

ECK0002, b0002, JW0001, Hs, thrA1, thrA2, thrD[1]

Product Desc.

ThrA[2][3];

Component of aspartate kinase I[2]

Aspartokinase I and homoserine dehydrogenase I, bifunctional[4]

Product Synonyms(s)

fused aspartokinase I[1], homoserine dehydrogenase I[1], B0002[2][1], ThrD[2][1], ThrA[2][1]

Function from GO

Biological Process

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): thrLABC[2], OP00248, thr, thrABC

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(thrA)    Textpresso(thrA)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

thrA

Mnemonic

Threonine

Synonyms

ECK0002, b0002, JW0001, Hs, thrA1, thrA2, thrD[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

0.01 minutes 

MG1655: 337..2799
gbrowse_img.php?coord=337..2799&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 336..2798
gbrowse_img.php?coord=336..2798&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 337..2799
gbrowse_img.php?coord=337..2799&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 337..2799
gbrowse_img.php?coord=337..2799&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 337..2799
gbrowse_img.php?coord=337..2799&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔthrA (Keio:JW0001)

deletion

deletion

PMID:16738554

Shigen

thrA34::Tn10

Insertion at 425 bp in MG1655 (NC_000913)

adapted from Nichols et al.

thrA34::Tn10

CGSC:4834

thrA1101

CGSC:5343

thrA1015

CGSC:5352

thrA1100

CGSC:5879

thrA3092::Tn10kan

PMID:2540407 PMID:9829956

CGSC:38986

thrA1016

CGSC:73731


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0001

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCGAGTGTTGAAGTTCGGCGG

Primer 2:CCGACTCCTAACTTCCATGAGAG

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[5]

est. P1 cotransduction: 28% [6]
Synonyms:car-96::Tn10

mdoB202::Tn10

Linked marker

CAG18430 = CGSC7488[5]

est. P1 cotransduction: 12% [6]
Synonyms:zjj-202::Tn10, zji-202::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10998

EchoBASE (EcoliWiki Page)

EchoBASE:EB0991

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0002

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0003

EcoGene (EcoliWiki Page)

EcoGene:EG10998

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:THRA

CGSC (EcoliWiki Page)

CGSC:111

ASAP (EcoliWikiPage)

ASAP:ABE-0000008


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ThrA

Synonyms

fused aspartokinase I[1], homoserine dehydrogenase I[1], B0002[2][1], ThrD[2][1], ThrA[2][1]

Product description

ThrA[2][3];

Component of aspartate kinase I[2]

Aspartokinase I and homoserine dehydrogenase I, bifunctional[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0016740

transferase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc [7]

complete

GO:0016597

amino acid binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002912

F

Seeded from EcoCyc [7]

complete

GO:0016491

oxidoreductase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0016301

kinase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0418

F

Seeded from EcoCyc [7]

complete

GO:0009088

threonine biosynthetic process

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0791

P

Seeded from EcoCyc [7]

complete

GO:0004412

homoserine dehydrogenase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000003

IEA: Inferred from Electronic Annotation

InterPro:IPR001342
InterPro:IPR011147
EC:1.1.1.3

F

Seeded from EcoCyc [7]

complete

GO:0004072

aspartate kinase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000003

IEA: Inferred from Electronic Annotation

InterPro:IPR001341
InterPro:IPR011147
EC:2.7.2.4

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0003824

catalytic activity

GO_REF:0000004
GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0021
SP_KW:KW-0511

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0005737

cytoplasm

C

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0005488

binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc [7]

complete

GO:0008152

metabolic process

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002912
InterPro:IPR016040

P

Seeded from EcoCyc [7]

complete

GO:0008652

amino acid biosynthetic process

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001048
InterPro:IPR001341
InterPro:IPR001342
InterPro:IPR018042
SP_KW:KW-0028

P

Seeded from EcoCyc [7]

complete

GO:0009067

aspartate family amino acid biosynthetic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011147

P

Seeded from EcoCyc [7]

complete

GO:0050661

NADP or NADPH binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005106

F

Seeded from EcoCyc [7]

complete

GO:0055114

oxidation reduction

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc [7]

complete

GO:0009086

methionine biosynthetic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0009090

homoserine_biosynthesis

PMID:11368768

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 12.5 [8]

GO:0009089

lysine_biosynthesis_via_diaminopimelate

PMID:11368768

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 12.5 [8]


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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of aspartate kinase I

could be indirect

Protein

glpD

PMID:16606699

Experiment(s):EBI-1135292

Protein

zwf

PMID:16606699

Experiment(s):EBI-1135292

Protein

ahpF

PMID:16606699

Experiment(s):EBI-1135292

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

Cytoplasm

PMID:4562989, PMID:354697, PMID:7003595, PMID:6777249

EchoLocation:thrA


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MRVLKFGGTS VANAERFLRV ADILESNARQ GQVATVLSAP AKITNHLVAM IEKTISGQDA
LPNISDAERI FAELLTGLAA AQPGFPLAQL KTFVDQEFAQ IKHVLHGISL LGQCPDSINA
ALICRGEKMS IAIMAGVLEA RGHNVTVIDP VEKLLAVGHY LESTVDIAES TRRIAASRIP
ADHMVLMAGF TAGNEKGELV VLGRNGSDYS AAVLAACLRA DCCEIWTDVD GVYTCDPRQV
PDARLLKSMS YQEAMELSYF GAKVLHPRTI TPIAQFQIPC LIKNTGNPQA PGTLIGASRD
EDELPVKGIS NLNNMAMFSV SGPGMKGMVG MAARVFAAMS RARISVVLIT QSSSEYSISF
CVPQSDCVRA ERAMQEEFYL ELKEGLLEPL AVTERLAIIS VVGDGMRTLR GISAKFFAAL
ARANINIVAI AQGSSERSIS VVVNNDDATT GVRVTHQMLF NTDQVIEVFV IGVGGVGGAL
LEQLKRQQSW LKNKHIDLRV CGVANSKALL TNVHGLNLEN WQEELAQAKE PFNLGRLIRL
VKEYHLLNPV IVDCTSSQAV ADQYADFLRE GFHVVTPNKK ANTSSMDYYH QLRYAAEKSR
RKFLYDTNVG AGLPVIENLQ NLLNAGDELM KFSGILSGSL SYIFGKLDEG MSFSEATTLA
REMGYTEPDP RDDLSGMDVA RKLLILARET GRELELADIE IEPVLPAEFN AEGDVAAFMA
NLSQLDDLFA ARVAKARDEG KVLRYVGNID EDGVCRVKIA EVDGNDPLFK VKNGENALAF
YSHYYQPLPL VLRGYGAGND VTAAGVFADL LRTLSWKLGV
Length

820

Mol. Wt

89.121 kDa

pI

5.5 (calculated)

Extinction coefficient

51,800 - 53,300 (calc based on 20 Y, 4 W, and 12 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:ASPKINIHOMOSERDEHYDROGI-MONOMER

UniProt (EcoliWiki Page)

UniProt:P00561

PFAM (EcoliWiki Page)

PFAM:PF00696

RefSeq (EcoliWiki Page)

RefSeq:NP_414543


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Notes

Links

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Expression

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===Overview===This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

thrLABC

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=317..357&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GenExpDB:b0002 (EcoliWiki Page)

Summary of data for thrA from multiple microarray studies

microarray

GEO Profiles:b0002 (EcoliWiki Page)

NCBI GEO profiles for thrA

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to thrA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10998

EchoBASE (EcoliWiki Page)

EchoBASE:EB0991

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0002

EcoGene (EcoliWiki Page)

EcoGene:EG10998


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Arabidopsis thaliana

  • AT4G19710 (score: 1.000; bootstrap: 100%)
  • AT1G31230 (score: 0.669)

From Inparanoid:20070104

Oryza gramene

  • P49079 (score: 1.000; bootstrap: 100%)
  • Q6ZD10 (score: 0.834)
  • Q69LG7 (score: 0.772)
  • P93402 (score: 0.768)
  • P49080 (score: 0.766)
  • Q6ZD09 (score: 0.644)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YJR139C (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPBC7763 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000017647 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

THRA

From SHIGELLACYC

E. coli O157

THRA

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00696


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Notes

Links

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References

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  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ 8.0 8.1 8.2 8.3 8.4 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

Categories

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