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thrB:On One Page

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Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

thrB

Gene Synonym(s)

ECK0003, b0003, JW0002[1]

Product Desc.

ThrB[2][3];

Component of homoserine kinase[2][3]

Homoserine kinase[4]

Product Synonyms(s)

homoserine kinase[1], B0003[2][1], ThrB[2][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): thrLABC[2], OP00248, thr, thrABC

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(thrB)    Textpresso(thrB)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

thrB

Mnemonic

Threonine

Synonyms

ECK0003, b0003, JW0002[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

0.06 minutes 

MG1655: 2801..3733
gbrowse_img.php?coord=2801..3733&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2800..3732
gbrowse_img.php?coord=2800..3732&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 2801..3733
gbrowse_img.php?coord=2801..3733&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2801..3733
gbrowse_img.php?coord=2801..3733&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 2801..3733
gbrowse_img.php?coord=2801..3733&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔthrB (Keio:JW0002)

deletion

deletion

PMID:16738554

Shigen
CGSC8333[5]

thrB1000

CGSC:5357

ΔthrB723::kan

PMID:16738554

CGSC:103150


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0002

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGTTAAAGTTTATGCCCCGGC

Primer 2:CCGTTTTCCAGTACTCGTGCGCC

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[5]

est. P1 cotransduction: 91% [6]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[5]

est. P1 cotransduction: 31% [6]
Synonyms:car-96::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10999

EchoBASE (EcoliWiki Page)

EchoBASE:EB0992

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0003

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0004

EcoGene (EcoliWiki Page)

EcoGene:EG10999

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:THRB

CGSC (EcoliWiki Page)

CGSC:110

ASAP (EcoliWikiPage)

ASAP:ABE-0000010


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ThrB

Synonyms

homoserine kinase[1], B0003[2][1], ThrB[2][1]

Product description

ThrB[2][3];

Component of homoserine kinase[2][3]

Homoserine kinase[4]

EC number (for enzymes) 
2.7.1.39[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0016740

transferase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc [7]

complete

GO:0016310

phosphorylation

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006204

P

Seeded from EcoCyc [7]

complete

GO:0016301

kinase activity

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR006204
SP_KW:KW-0418

F

Seeded from EcoCyc [7]

complete

GO:0009088

threonine biosynthetic process

GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

SP_KW:KW-0791
HAMAP:MF_00384

P

Seeded from EcoCyc 12.5 [8]

complete

GO:0008652

amino acid biosynthetic process

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0028

P

Seeded from EcoCyc [7]

complete

GO:0006566

threonine metabolic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000870

P

Seeded from EcoCyc [7]

complete

GO:0005737

cytoplasm

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc 12.5 [8]

complete

GO:0005524

ATP binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR000870
InterPro:IPR006203
InterPro:IPR006204
SP_KW:KW-0067
HAMAP:MF_00384

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0004413

homoserine kinase activity

GO_REF:0000002
GO_REF:0000003
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR000870
EC:2.7.1.39
HAMAP:MF_00384

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0000166

nucleotide binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc [7]

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of homoserine kinase

could be indirect

Protein

groL

PMID:16606699

Experiment(s):EBI-1135297

Protein

glpD

PMID:16606699

Experiment(s):EBI-1135297

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MVKVYAPASS ANMSVGFDVL GAAVTPVDGA LLGDVVTVEA AETFSLNNLG RFADKLPSEP
RENIVYQCWE RFCQELGKQI PVAMTLEKNM PIGSGLGSSA CSVVAALMAM NEHCGKPLND
TRLLALMGEL EGRISGSIHY DNVAPCFLGG MQLMIEENDI ISQQVPGFDE WLWVLAYPGI
KVSTAEARAI LPAQYRRQDC IAHGRHLAGF IHACYSRQPE LAAKLMKDVI AEPYRERLLP
GFRQARQAVA EIGAVASGIS GSGPTLFALC DKPETAQRVA DWLGKNYLQN QEGFVHICRL
DTAGARVLEN
Length

310

Mol. Wt

33.623 kDa

pI

5.5 (calculated)

Extinction coefficient

33,920 - 35,045 (calc based on 8 Y, 4 W, and 9 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:HOMOSERKIN-MONOMER

UniProt (EcoliWiki Page)

UniProt:P00547

PFAM (EcoliWiki Page)

PFAM:PF00288

RefSeq (EcoliWiki Page)

RefSeq:NP_414544


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

thrLABC

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=2781..2821&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b0003 (EcoliWiki Page)

NCBI GEO profiles for thrB

microarray

GenExpDB:b0003 (EcoliWiki Page)

Summary of data for thrB from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to thrB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10999

EchoBASE (EcoliWiki Page)

EchoBASE:EB0992

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0003

EcoGene (EcoliWiki Page)

EcoGene:EG10999


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Arabidopsis thaliana

  • AT2G17265 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q6K969 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YHR025W (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPBC4C33 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

THRB

From SHIGELLACYC

E. coli O157

THRB

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00288


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ 8.0 8.1 8.2 8.3 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

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