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thrL:On One Page

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Quickview | Gene | Product(s) | Expression| Evolution | References |

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See Help:Quickview for help with entering information in the Quickview table.

Standard Name

thrL

Gene Synonym(s)

ECK0001, b0001, JW4367[1]

Product Desc.

thr operon leader peptide[2][3]

Regulatory leader peptide for thrABC operon[4]

Product Synonyms(s)

thr operon leader peptide[1], B0001[2][1], ThrL[2][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): thrLABC[2], OP00248, thr, thrABC

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(thrL)    Textpresso(thrL)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

thrL

Mnemonic

Threonine

Synonyms

ECK0001, b0001, JW4367[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

0 minutes 

MG1655: 190..255
gbrowse_img.php?coord=190..255&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 190..255
gbrowse_img.php?coord=190..255&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 190..255
gbrowse_img.php?coord=190..255&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 190..255
gbrowse_img.php?coord=190..255&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 190..255
gbrowse_img.php?coord=190..255&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔthrL (Keio:JW4367)

deletion

deletion

PMID:16738554

Shigen

ΔthrL721::kan

PMID:16738554

CGSC:103154


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[5]

est. P1 cotransduction: 99% [6]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[5]

est. P1 cotransduction: 28% [6]
Synonyms:car-96::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11277

EchoBASE (EcoliWiki Page)

EchoBASE:EB1255

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0001

EcoGene (EcoliWiki Page)

EcoGene:EG11277

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:THRL

ASAP (EcoliWikiPage)

ASAP:ABE-0000006

ASAP (EcoliWikiPage)

ASAP:ABE-0000006


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ThrL

Synonyms

thr operon leader peptide[1], B0001[2][1], ThrL[2][1]

Product description

thr operon leader peptide[2][3]

Regulatory leader peptide for thrABC operon[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0009088

threonine biosynthetic process

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR011720
SP_KW:KW-0791

P

Seeded from EcoCyc 12.5 [7]

complete

GO:0008652

amino acid biosynthetic process

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0028

P

Seeded from EcoCyc [8]

complete

GO:0031556

transcriptional attenuation by ribosome

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01907

P

Seeded from EcoCyc [8]

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MKRISTTITT TITITTGNGA G
Length

21

Mol. Wt

2.138 kDa

pI

11.8 (calculated)

Extinction coefficient

0 (calc based on Y, W, and C residues)

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Domains/Motifs/Modification Sites See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11277-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0AD86

RefSeq (EcoliWiki Page)

RefSeq:NP_414542


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

thrLABC

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=170..210&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

Notes

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b0001 (EcoliWiki Page)

NCBI GEO profiles for thrL

microarray

GenExpDB:b0001 (EcoliWiki Page)

Summary of data for thrL from multiple microarray studies

microarray

GEO Profiles:b0001 (EcoliWiki Page)

NCBI GEO profiles for thrL

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to thrL Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11277

EchoBASE (EcoliWiki Page)

EchoBASE:EB1255

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0001

EcoGene (EcoliWiki Page)

EcoGene:EG11277


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Notes

Links

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Evolution

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Homologs in Other Organisms

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Do-It-Yourself Web Tools

Notes

Families

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

E. coli O157

THRL

From ECOO157CYC

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  8. ↑ 8.0 8.1 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70

Categories

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