thrS:Gene Product(s)

Standard name	ThrS
Synonyms	threonyl-tRNA synthetase^[1], B1719^[2]^[1], ThrS^[2]^[1]
Product description	threonyl-tRNA synthetase^[2]^[3]; Component of threonyl-tRNA synthetase^[2]^[3] Threonine--tRNA ligase, autogenously regulated; binds Zn(II)^[4]
EC number (for enzymes)	6.1.1.3^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0046872	metal ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc ^[5]	complete
	GO:0008270	zinc ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0862	F	Seeded from EcoCyc ^[5]	complete
	GO:0006435	threonyl-tRNA aminoacylation	GO_REF:0000002 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR002320 HAMAP:MF_00184	P	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005737	cytoplasm	GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR002314 InterPro:IPR002320 InterPro:IPR006195 SP_KW:KW-0963	C	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005524	ATP binding	GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000004 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR002314 InterPro:IPR002320 InterPro:IPR004154 InterPro:IPR006195 SP_KW:KW-0067 HAMAP:MF_00184	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0004829	threonine-tRNA ligase activity	GO_REF:0000002 GO_REF:0000003 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR002320 EC:6.1.1.3 HAMAP:MF_00184	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0003723	RNA binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0694	F	Seeded from EcoCyc ^[5]	complete
	GO:0000166	nucleotide binding	GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR002314 InterPro:IPR002320 InterPro:IPR006195 SP_KW:KW-0547	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0000900	translation repressor activity, nucleic acid binding	PMID:2207165^[7]	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc ^[5]	complete
	GO:0004812	aminoacyl-tRNA ligase activity	GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR002314 InterPro:IPR004154 InterPro:IPR006195 SP_KW:KW-0030	F	Seeded from EcoCyc ^[5]	complete
	GO:0006412	translation	GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR002314 InterPro:IPR002320 InterPro:IPR004154 InterPro:IPR006195 InterPro:IPR012947 InterPro:IPR018158 SP_KW:KW-0648	P	Seeded from EcoCyc ^[5]	complete
	GO:0006417	regulation of translation	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0810	P	Seeded from EcoCyc ^[5]	complete
	GO:0016874	ligase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0436	F	Seeded from EcoCyc ^[5]	complete
	GO:0045947	negative regulation of translational initiation	PMID:3930755^[8]	IMP: Inferred from Mutant Phenotype		P	Seeded from EcoCyc ^[5]	complete
	GO:0030371	translation repressor activity				F	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0005524	ATP binding	PMID:10319817^[9]	IDA: Inferred from Direct Assay		F		complete
	GO:0003723	RNA binding	PMID:10319817^[9]	IDA: Inferred from Direct Assay		F		complete
	GO:0008270	zinc ion binding	PMID:10319817^[9]	IMP: Inferred from Mutant Phenotype		F		complete
	GO:0005515	protein binding	PMID:10319817^[9]	IPI: Inferred from Physical Interaction	EcoliWiki:thrS	F	ThrRS is a homodimer of thrS gene products.	complete
	GO:0006435	threonyl-tRNA aminoacylation	PMID:330505^[10]	IDA: Inferred from Direct Assay		P		complete
	GO:0005737	cytoplasm	PMID:330505^[10]	IDA: Inferred from Direct Assay		C		complete
	GO:0004829	threonine-tRNA ligase activity	PMID:330505^[10]	IDA: Inferred from Direct Assay		F		complete
	GO:0016876	ligase activity, forming aminoacyl-tRNA and related compounds	PMID:330505^[10]	IDA: Inferred from Direct Assay		F		complete
	GO:0043039	tRNA aminoacylation	PMID:330505^[10]	IDA: Inferred from Direct Assay		P		complete
	GO:0006418	tRNA aminoacylation for protein translation	PMID:330505^[10]	IDA: Inferred from Direct Assay		P		complete
	GO:0008144	drug binding	PMID:330505^[10]	IDA: Inferred from Direct Assay		F	Borrelidin	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of threonyl-tRNA synthetase	could be indirect
Protein	rplA		PMID:15690043^[11]	Experiment(s):EBI-879795, EBI-884548
Protein	dnaK		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	eno		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	ibpA		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	rpe		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	rplC		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	rplI		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	rplM		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	rplS		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	rplV		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	rpsA		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	rpsB		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	rpsE		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	rpsF		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	rpsG		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	rpsJ		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	rpsM		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	rpsN		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	rpsT		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	wcaB		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	yhbY		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	yicC		PMID:15690043^[11]	Experiment(s):EBI-884548
Protein	recR		PMID:16606699^[12]	Experiment(s):EBI-1140596
Protein	nadE		PMID:16606699^[12]	Experiment(s):EBI-1140596
Protein	rpsC		PMID:16606699^[12]	Experiment(s):EBI-1140596
Protein	yggM		PMID:16606699^[12]	Experiment(s):EBI-1140596
Small Molecule	Borrelidin		PMID:330505^[10]	See figure 4.
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Notes

Localization

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Compartment	Description	Evidence	Source	Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence	at EcoCyc MPVITLPDGS QRHYDHAVSP MDVALDIGPG LAKACIAGRV NGELVDACDL IENDAQLSII TAKDEEGLEI IRHSCAHLLG HAIKQLWPHT KMAIGPVIDN GFYYDVDLDR TLTQEDVEAL EKRMHELAEK NYDVIKKKVS WHEARETFAN RGESYKVSIL DENIAHDDKP GLYFHEEYVD MCRGPHVPNM RFCHHFKLMK TAGAYWRGDS NNKMLQRIYG TAWADKKALN AYLQRLEEAA KRDHRKIGKQ LDLYHMQEEA PGMVFWHNDG WTIFRELEVF VRSKLKEYQY QEVKGPFMMD RVLWEKTGHW DNYKDAMFTT SSENREYCIK PMNCPGHVQI FNQGLKSYRD LPLRMAEFGS CHRNEPSGSL HGLMRVRGFT QDDAHIFCTE EQIRDEVNGC IRLVYDMYST FGFEKIVVKL STRPEKRIGS DEMWDRAEAD LAVALEENNI PFEYQLGEGA FYGPKIEFTL YDCLDRAWQC GTVQLDFSLP SRLSASYVGE DNERKVPVMI HRAILGSMER FIGILTEEFA GFFPTWLAPV QVVIMNITDS QSEYVNELTQ KLSNAGIRVK ADLRNEKIGF KIREHTLRRV PYMLVCGDKE VESGKVAVRT RRGKDLGSMD VNEVIEKLQQ EIRSRSLKQL EE
Length	642
Mol. Wt	74.014 kDa
pI	6.1 (calculated)
Extinction coefficient	96,260 - 97,885 (calc based on 24 Y, 11 W, and 13 C residues)
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Domains/Motifs/Modification Sites

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Type	Residues	Description	Notes	References
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Structure
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Structures

Representative Escherichia coli structure:
1EVK|A
1-401 of 642 residues (E-value: 2e-221) (Percent Identity: 100.00)
View the PDB Table for thrS

Models

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:THRS-MONOMER
UniProt (EcoliWiki Page)	UniProt:P0A8M3
PFAM (EcoliWiki Page)	PFAM:PF02824
RefSeq (EcoliWiki Page)	RefSeq:NP_416234
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 ^5.4 ^5.5 ^5.6 ^5.7 ^5.8 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
↑ ^6.0 ^6.1 ^6.2 ^6.3 ^6.4 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Moine H et al. (1990) The translational regulation of threonyl-tRNA synthetase. Functional relationship between the enzyme, the cognate tRNA and the ribosome. Biochim Biophys Acta 1050: 343-50 PubMed EcoliWiki page
↑ Springer M et al. (1985) Autogenous control of Escherichia coli threonyl-tRNA synthetase expression in vivo. J Mol Biol 185: 93-104 PubMed EcoliWiki page
↑ ^9.0 ^9.1 ^9.2 ^9.3 Sankaranarayanan R et al. (1999) The structure of threonyl-tRNA synthetase-tRNA(Thr) complex enlightens its repressor activity and reveals an essential zinc ion in the active site. Cell 97: 371-81 PubMed EcoliWiki page
↑ ^10.0 ^10.1 ^10.2 ^10.3 ^10.4 ^10.5 ^10.6 ^10.7 Hennecke H et al. (1977) Threonyl-transfer ribonucleic acid synthetase from Escherichia coli: subunit structure and genetic analysis of the structural gene by means of a mutated enzyme and of a specialized transducing lambda bacteriophage. J Bacteriol 131: 943-50 PubMed EcoliWiki page
↑ ^11.00 ^11.01 ^11.02 ^11.03 ^11.04 ^11.05 ^11.06 ^11.07 ^11.08 ^11.09 ^11.10 ^11.11 ^11.12 ^11.13 ^11.14 ^11.15 ^11.16 ^11.17 ^11.18 ^11.19 ^11.20 ^11.21 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
↑ ^12.0 ^12.1 ^12.2 ^12.3 Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page

thrS:Gene Product(s)

From EcoliWiki

Nomenclature

Notes

Function

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Localization

Notes

Structure and Physical Properties

Structure figures

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Gene Product Resources

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Accessions in Other Databases

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References

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