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Standard Name


Gene Synonym(s)

trmU, ECK1119, b1133, JW1119, asuE, ycfB[1][2], ycfB

Product Desc.

tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase[3][2]

tRNA U34 sulfurtransferase; first step in mnm(5)-s(2)U34-tRNA synthesis; thiolates U34 at C2; YccK(TusE) binding partner; antisuppressor[4]

Product Synonyms(s)

tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase[1], B1133[3][1], YcfB[3][1], AsuE[3][1], TrmU[3][1], MnmA[3][1] , asuE, ECK1119, JW1119, trmU, ycfB, b1133

Function from GO
Knock-Out Phenotype

transcription unit(s):

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Sulfur may be donated to tRNA U34 from YccK(TusD)-activated MnmA or from YccK(TusD) directly. Arigoni(1998) reported that mnmA(ycfB) is an essential gene, however Dassain(1999) and Kambampati(2003) both found mnmA(ycfB) deletion mutants to grow slowly.[4]


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  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.