trpC:Gene - EcoliWiki
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trpC:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

trpC

Mnemonic
Synonyms

ECK1256, b1262, JW1254, trpF, trpCF[1][2]

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Notes

Location(s) and DNA Sequence

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StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

28.37 minutes 

MG1655: 1317809..1316451
gbrowse_img.php?coord=1315451..1318809&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 1321499..1320141
gbrowse_img.php?coord=1319141..1322499&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔtrpC (Keio:JW1254)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9131[4]

trpC83::Tn10

Insertion at 1,317,596 bp in MG1655 (NC_000913)

adapted from Nichols et al.[5]

CAG18455 = CGSC7371[4]

Synonyms: trpB83::Tn10

trpC83::Tn10

PMID:9829956[6]

CGSC:4709

trpC45

CGSC:4746

trpC60

CGSC:6951

trpC9999

CGSC:27412

trpC3117::Tn10kan

PMID:2540407[7] PMID:9829956[6]

CGSC:39838

trpC3193::Tn10kan

PMID:2540407[7] PMID:9829956[6]

CGSC:46774

trpC9800

PMID:4945859[8]

CGSC:58648

trpC9830

PMID:4945859[8]

CGSC:58650

trpC9941

PMID:5339605[9]

CGSC:65101

ΔtrpC720

CGSC:69381

ΔtrpC721(trpF)

PMID:9605328[10] PMID:7556082[11]

CGSC:69387

ΔtrpC770::kan

PMID:16738554[3]

CGSC:103226


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Notes

Genetic Resources

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ResourceResource TypeSourceNotes/Reference

JW1254

Plasmid clone

Shigen

PMID:16769691[12]

Status:Clone OK

Primer 1:GCCCAAACCGTTTTAGCGAAAAT

Primer 2:CCATATGCGCGCAGCGTCTGGAA

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[4]

est. P1 cotransduction: 64% [5]

Synonyms:zch-506::Tn10, zci-506::Tn10

zcj-233::Tn10

Linked marker

CAG12028 = CGSC7373[4]

est. P1 cotransduction: 8% [5]

Synonyms:zci-233::Tn10

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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11026

EchoBASE (EcoliWiki Page)

EchoBASE:EB1019

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1262

EcoGene (EcoliWiki Page)

EcoGene:EG11026

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:TRPC

CGSC (EcoliWiki Page)

CGSC:72

ASAP (EcoliWikiPage)

ASAP:ABE-0004238


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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  4. 4.0 4.1 4.2 4.3 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 5.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. 6.0 6.1 6.2 Nichols BP et al. (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J Bacteriol 180: 6408-11 PubMed EcoliWiki page
  7. 7.0 7.1 Singer M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol Rev 53: 1-24 PubMed EcoliWiki page
  8. 8.0 8.1 Yanofsky C et al. (1971) Polarity and enzyme functions in mutants of the first three genes of the tryptophan operon of Escherichia coli. Genetics 69: 409-33 PubMed EcoliWiki page
  9. Yanofsky C & Ito J (1966) Nonsense codons and polarity in the tryptophan operon. J Mol Biol 21: 313-34 PubMed EcoliWiki page
  10. Darimont B et al. (1998) Mutational analysis of the active site of indoleglycerol phosphate synthase from Escherichia coli. Protein Sci 7: 1221-32 PubMed EcoliWiki page
  11. Sterner R et al. (1995) (Beta alpha)8-barrel proteins of tryptophan biosynthesis in the hyperthermophile Thermotoga maritima. EMBO J 14: 4395-402 PubMed EcoliWiki page
  12. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page

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