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tsx:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tsx

Mnemonic

T-six

Synonyms

ECK0405, b0411, JW0401, T6rec, nupA[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

9.28 minutes 

MG1655: 431237..430353
gbrowse_img.php?coord=430353..431237&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 400435..399662
gbrowse_img.php?coord=399662..400435&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 431237..430353
gbrowse_img.php?coord=430353..431237&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 370568..369684
gbrowse_img.php?coord=369684..370568&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 333112..333996
gbrowse_img.php?coord=333112..333996&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

Δtsx (Keio:JW0401)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8574[3]

tsxN276Y

N276Y

(in phage resistant mutant)

Strain variation; seeded from UniProt:P0A927

tsx-247::Tn10

Insertion at 430,667 bp in MG1655 (NC_000913)

adapted from Nichols et al.[4]

CAG12148 = CGSC7337[3]

Synonyms:

tsx-33

CGSC:4496

tsx-29

CGSC:4846

tsx-67

CGSC:4930

tsx-1

CGSC:5240

tsx-70

CGSC:5297

tsx-358

CGSC:5309

tsx-81

CGSC:5575

tsx-76

CGSC:5602

tsx-79

CGSC:5608

tsx-84

CGSC:5663

tsx-95

CGSC:5684

tsx-7

CGSC:5796

tsx-63

CGSC:5830

tsx-78

CGSC:5945

tsx-96

CGSC:6184

tsx-71

CGSC:6370

tsx-64

CGSC:6458

tsx-273

CGSC:6598

tsx-97

CGSC:6623

tsx-0

CGSC:6729

tsx-68

CGSC:7094

tsx-463

CGSC:7525

tsx-352

CGSC:7563

tsx-83

CGSC:7609

tsx-80

CGSC:7845

tsx-82

CGSC:7912

tsx-65

CGSC:8002

tsx-354

CGSC:8220

tsx-356

CGSC:8224

tsx-357

CGSC:8227

tsx-3

CGSC:8257

tsx-61

CGSC:8309

tsx-59

CGSC:8355

tsx-6

CGSC:8384

tsx-274

CGSC:8505

tsx-87

CGSC:8763

tsx-23

CGSC:8896

tsx-25

CGSC:8897

tsx-464

CGSC:9000

tsx-36

CGSC:9084

tsx-57

CGSC:9107

tsx-5

CGSC:9357

tsx-353

CGSC:9453

tsx-462::Tn10

CGSC:9490

tsx-93

CGSC:9803

tsx-466

CGSC:9983

tsx-69

CGSC:10430

tsx-465(Am)

CGSC:10863

amber (UAG) mutation

tsx-72

CGSC:11129

tsx-21

CGSC:11149

tsx-2

CGSC:11151

tsx-85

CGSC:11842

tsx-460

CGSC:12214

tsx-18

CGSC:13122

tsx-17

CGSC:13168

tsx-27

CGSC:13213

tsx-4

CGSC:13243

tsx-58

CGSC:13378

tsx-62

CGSC:13948

tsx-73

CGSC:14208

tsx-459

CGSC:14332

tsx-19

CGSC:15762

tsx-37

CGSC:16094

tsx-77

CGSC:17075

tsx-247::Tn10

CGSC:39365

tsx-3100::Tn10kan

CGSC:39368

tsx-35

CGSC:43389

tsx-74

CGSC:72463

tsx-75

CGSC:72484

Δtsx-773::kan

PMID:16738554[2]

CGSC:103248


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0401

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAAAAAAACATTACTGGCAGC

Primer 2:CCGAAGTTGTAACCTACTACCAG

zai-3053::Tn10

Linked marker

CAG18091 = CGSC7336[3]

est. P1 cotransduction: 23% [4]
Synonyms:zaj-3053::Tn10 nnnCAG18091 also carries proC9999 (CGSC).

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[3]

est. P1 cotransduction: 33% [4]
Synonyms:zba-3054::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11035

EchoBASE (EcoliWiki Page)

EchoBASE:EB1028

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0411

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0521

EcoGene (EcoliWiki Page)

EcoGene:EG11035

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:TSX

CGSC (EcoliWiki Page)

CGSC:62

ASAP (EcoliWikiPage)

ASAP:ABE-0001425


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. ↑ 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  5. ↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page

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