tufA:Gene Product(s)

Standard name	protein chain elongation factor EF-Tu
Synonyms	protein chain elongation factor EF-Tu (duplicate of tufB)^[1], protein chain elongation factor EF-Tu (duplicate of tufA)^[1], B3339^[2]^[1], PulT^[2]^[1], KirT^[2]^[1], TufA^[2]^[1], EF-Tu^[2]^[1]
Product description	elongation factor Tu^[2]^[3] EF-Tu, Elongation Factor-Translation, unstable; GTP-dependent binding of aa-tRNA to the A-site of ribosomes; has intrinsic GTPase activity when bound to kirromycin^[4]
EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0046677	response to antibiotic	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0046	P	Seeded from EcoCyc ^[5]	complete
	GO:0016020	membrane	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc ^[5]	complete
	GO:0006414	translational elongation	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004541	P	Seeded from EcoCyc ^[5]	complete
	GO:0006412	translation	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0648	P	Seeded from EcoCyc ^[5]	complete
	GO:0005737	cytoplasm	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005525	GTP binding	GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000004 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR000795 InterPro:IPR004160 InterPro:IPR004161 InterPro:IPR004541 InterPro:IPR005225 SP_KW:KW-0342 HAMAP:MF_00118	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0003924	GTPase activity	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000795	F	Seeded from EcoCyc ^[5]	complete
	GO:0003746	translation elongation factor activity	GO_REF:0000002 GO_REF:0000004 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR004541 SP_KW:KW-0251 HAMAP:MF_00118	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0000166	nucleotide binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc ^[5]	complete
	GO:0006970	response to osmotic stress				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0005515	protein binding	PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7]	IPI: Inferred from Physical Interaction	UniProtKB:P00956 UniProtKB:P04951 UniProtKB:P0A6P1 UniProtKB:P0A6Y5 UniProtKB:P0A6Z6 UniProtKB:P0A8J4 UniProtKB:P0A959 UniProtKB:P0ADX9 UniProtKB:P0AFQ7 UniProtKB:P0AG30 UniProtKB:P10441 UniProtKB:P15038 UniProtKB:P17888 UniProtKB:P22634 UniProtKB:P23367 UniProtKB:P23909 UniProtKB:P30750 UniProtKB:P33590 UniProtKB:P39408 UniProtKB:P61517 UniProtKB:P63389 UniProtKB:P76251 UniProtKB:P77756	F	Seeded from EcoCyc ^[5]	complete
	GO:0005622	intracellular	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004541	C	Seeded from EcoCyc ^[5]	complete
	GO:0005886	plasma membrane	GO_REF:0000004 GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003 SP_SL:SL-0039	C	Seeded from EcoCyc ^[5]	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	tsf	PMID:16606699^[8]	Experiment(s):EBI-1145593, EBI-1145593
Protein	acrF	PMID:15690043^[7]	Experiment(s):EBI-894882
Protein	fbpC	PMID:15690043^[7]	Experiment(s):EBI-894882
Protein	ydhQ	PMID:15690043^[7]	Experiment(s):EBI-894882
Protein	rpsB	PMID:15690043^[7]	Experiment(s):EBI-894882
Protein	valS	PMID:15690043^[7]	Experiment(s):EBI-894882
Protein	xylA	PMID:15690043^[7]	Experiment(s):EBI-894882
Protein	yaaA	PMID:15690043^[7]	Experiment(s):EBI-894882
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Source	Notes
Cytoplasm		PMID:7525272^[9], PMID:7021545^[10]	EchoLocation:tufA
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence	at EcoCyc MSKEKFERTK PHVNVGTIGH VDHGKTTLTA AITTVLAKTY GGAARAFDQI DNAPEEKARG ITINTSHVEY DTPTRHYAHV DCPGHADYVK NMITGAAQMD GAILVVAATD GPMPQTREHI LLGRQVGVPY IIVFLNKCDM VDDEELLELV EMEVRELLSQ YDFPGDDTPI VRGSALKALE GDAEWEAKIL ELAGFLDSYI PEPERAIDKP FLLPIEDVFS ISGRGTVVTG RVERGIIKVG EEVEIVGIKE TQKSTCTGVE MFRKLLDEGR AGENVGVLLR GIKREEIERG QVLAKPGTIK PHTKFESEVY ILSKDEGGRH TPFFKGYRPQ FYFRTTDVTG TIELPEGVEM VMPGDNIKMV VTLIHPIAMD DGLRFAIREG GRTVGAGVVA KVLG
Length	394
Mol. Wt	43.283 kDa
pI	5.3 (calculated)
Extinction coefficient	20,400 - 20,775 (calc based on 10 Y, 1 W, and 3 C residues)
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Domains/Motifs/Modification Sites

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Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P0A6N1
Modification Site	62	phosphorylation site at T62	probability greater than 75%	PMID:17938405^[11]
Modification Site	159	phosphorylation site at S159	probability greater than 75%	PMID:17938405^[11]
Modification Site	168	phosphorylation site at T168	probability less than 75%	PMID:17938405^[11]
Modification Site	66	phosphorylation site at S66	probability less than 75%	PMID:17938405^[11]
Modification Site	174	phosphorylation site at S174	probability less than 75%	PMID:17938405^[11]
Modification Site	222	phosphorylation site at S222	probability greater than 75%	PMID:17938405^[11]
Modification Site	65	phosphorylation site at T65	probability less than 75%	PMID:17938405^[11]
Modification Site	313	phosphorylation site at S313	probability greater than 75%	PMID:17938405^[11]
Modification Site	332	phosphorylation site at Y332	probability greater than 75%	PMID:17938405^[11]
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

Representative Escherichia coli structure:
1D8T|A
1-393 of 394 residues (E-value: 6e-211) (Percent Identity: 100.00)
View the PDB Table for tufA

Models

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Structure figures

Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG11036-MONOMER
UniProt (EcoliWiki Page)	UniProt:P0A6N1
PFAM (EcoliWiki Page)	PFAM:PF00009
RefSeq (EcoliWiki Page)	RefSeq:NP_417798
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 ^5.4 ^5.5 ^5.6 ^5.7 ^5.8 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
↑ ^6.0 ^6.1 ^6.2 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^7.00 ^7.01 ^7.02 ^7.03 ^7.04 ^7.05 ^7.06 ^7.07 ^7.08 ^7.09 ^7.10 ^7.11 ^7.12 ^7.13 ^7.14 ^7.15 ^7.16 ^7.17 ^7.18 ^7.19 ^7.20 ^7.21 ^7.22 ^7.23 ^7.24 ^7.25 ^7.26 ^7.27 ^7.28 ^7.29 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
↑ Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
↑ Mesters JR et al. (1994) The structural and functional basis for the kirromycin resistance of mutant EF-Tu species in Escherichia coli. EMBO J 13: 4877-85 PubMed EcoliWiki page
↑ Laursen RA et al. (1981) The amino acid sequence of elongation factor Tu of Escherichia coli. The complete sequence. J Biol Chem 256: 8102-9 PubMed EcoliWiki page
↑ ^11.0 ^11.1 ^11.2 ^11.3 ^11.4 ^11.5 ^11.6 ^11.7 ^11.8 Macek B et al. (2007) Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation. Mol Cell Proteomics PubMed EcoliWiki page

tufA:Gene Product(s)

From EcoliWiki

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

References

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