ybhA:Gene Product(s)

Standard name	YbhA
Synonyms	predicted hydrolase^[1], YbhA^[2]^[1], B0766^[2]^[1], fructose 1,6-bisphosphatase III^[2]^[1]
Product description	EG11239^[2]^[3] Pyridoxal phosphate (PLP) phosphatase; phosphoramidase; physiological role unknown; HAD14^[4]
EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0046872	metal ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc ^[5]	complete
	GO:0016787	hydrolase activity	GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR000150 SP_KW:KW-0378	F	Seeded from EcoCyc ^[5]	complete
Deprecated	GO:0016020	membrane	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001757	C	No membrane spanning segments were predicted for YbhA using TMHMM 3.0. This electronic annotation probably made because YbhA shares sequence similarity with the catalytic domain of P-type ATPases.	complete
Deprecated	GO:0015662	ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001757	F	YbhA has sequence similarity to only the catalytic domain of P-type ATPases. No predicted membrane spanning regions were identified in YbhA using TMHMM 3.0.	complete
	GO:0008152	metabolic process	GO_REF:0000002 GO_REF:0000002 GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000150 InterPro:IPR005834 InterPro:IPR006379	P	Seeded from EcoCyc ^[5]	complete
	GO:0006810	transport	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001757	P	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005524	ATP binding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001757	F	Seeded from EcoCyc ^[5]	complete
	GO:0003824	catalytic activity	GO_REF:0000002 GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005834 InterPro:IPR006379	F	Seeded from EcoCyc ^[5]	complete
	GO:0000287	magnesium ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460	F	Seeded from EcoCyc ^[5]	complete
	GO:0042822	pyridoxal phosphate metabolic process				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0006754	ATP biosynthetic process	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001757	P	Seeded from EcoCyc ^[5]	complete
Under review	GO:0042132	fructose 1,6-bisphosphate 1-phosphatase activity	PMID:16990279^[7]			F	YbhA releases phosphate from fructose-1,6-bis-phosphate, but which phosphate is released wasn't determined.	required field missing
	GO:0050308	sugar-phosphatase activity	PMID:16990279^[7]	IDA: Inferred from Direct Assay		F		complete
	GO:0050308	sugar-phosphatase activity	PMID:16889420^[8]	IDA: Inferred from Direct Assay		F		complete
	GO:0016773	phosphotransferase activity, alcohol group as acceptor	PMID:16889420^[8]	IDA: Inferred from Direct Assay		F	Purified YbhA will form glycerol phosphate from glycerol and inorganic phosphate (Table 4). The authors note that the phosphotransferase activity is lower than the phosphatase activity, and that other mono-phosphates can serve as the phosphate donor.	complete
	GO:0033883	pyridoxal phosphatase activity	PMID:16990279^[7]	IDA: Inferred from Direct Assay		F	Figure 1 and Table 2.	complete
	GO:0016791	phosphatase activity	PMID:16990279^[7]	IDA: Inferred from Direct Assay		F	YbhA had phosphatase activity on 8 of the 80 phosphorylated compounds tested. These were (in decreasing order of activity): pyridoxal-5-P, erythrose-4-P, Fructose-1,6-bis-P, flavin mononucleotide (FMN), thiamine pyrophosphate, phosphoribosyl pyrophosphate (PRPP), Glucose-1,6-di-P, and 6-phosphogluconate (Fig. 1).	complete
	GO:0000287	magnesium ion binding	PMID:16990279^[7]	IDA: Inferred from Direct Assay		F	Km is 0.14±0.02 mM with pyridoxal-5-phosphate as the substrate (Suppl. Table 2).	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	groL	PMID:16606699^[9]	Experiment(s):EBI-1137896
Protein	moaA	PMID:16606699^[9]	Experiment(s):EBI-1137896
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Notes

Localization

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Compartment	Description	Evidence	Source	Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence	at EcoCyc MTTRVIALDL DGTLLTPKKT LLPSSIEALA RAREAGYQLI IVTGRHHVAI HPFYQALALD TPAICCNGTY LYDYHAKTVL EADPMPVIKA LQLIEMLNEH HIHGLMYVDD AMVYEHPTGH VIRTSNWAQT LPPEQRPTFT QVASLAETAQ QVNAVWKFAL THDDLPQLQH FGKHVEHELG LECEWSWHDQ VDIARGGNSK GKRLTKWVEA QGWSMENVVA FGDNFNDISM LEAAGTGVAM GNADDAVKAR ANIVIGDNTT DSIAQFIYSH LI
Length	272
Mol. Wt	30.2 kDa
pI	5.8 (calculated)
Extinction coefficient	44,920 - 45,295 (calc based on 8 Y, 6 W, and 3 C residues)
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Domains/Motifs/Modification Sites

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Type	Residues	Description	Notes	References
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Structure
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Structures

View the PDB Table for ybhA

Models

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Structure figures

Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG11239-MONOMER
UniProt (EcoliWiki Page)	UniProt:P21829
PFAM (EcoliWiki Page)	PFAM:PF00702
RefSeq (EcoliWiki Page)	RefSeq:NP_415287
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 ^5.4 ^5.5 ^5.6 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
↑ EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^7.0 ^7.1 ^7.2 ^7.3 ^7.4 Kuznetsova E et al. (2006) Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family. J Biol Chem 281: 36149-61 PubMed EcoliWiki page
↑ ^8.0 ^8.1 Saito N et al. (2006) Metabolomics approach for enzyme discovery. J Proteome Res 5: 1979-87 PubMed EcoliWiki page
↑ ^9.0 ^9.1 Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page

ybhA:Gene Product(s)

From EcoliWiki

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

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Accessions in Other Databases

Notes

Links

References

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