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yigL:Gene Product(s)
From EcoliWiki
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
YigL |
|---|---|
| Synonyms | |
| Product description |
Pyridoxal phosphate (PLP) phosphatase; weak phosphoramidase; additional in vitro substrates include: 2-deoxyglucose-6-P, glucose-6-P; physiological role unknown; HAD16[4] |
| EC number (for enzymes) |
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| edit table |
Notes
HAD indicates that YigL belongs to the haloacid dehalogenase-like phosphatase family.
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference(s) | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016787 |
hydrolase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [5] |
complete | |||
| GO:0008152 |
metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [5] |
complete | |||
| GO:0003824 |
catalytic activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [5] |
complete | |||
| GO:0042822 |
pyridoxal phosphate metabolic process |
P |
Seeded from EcoCyc 11.1[3]. |
required fields missing | ||||
| GO:0050308 |
sugar-phosphatase activity |
IDA: Inferred from Direct Assay |
F |
In vitro substrates were glucose-6-phosphate and 2-deoxyglucose-6-phosphate (Figure 1). |
complete | |||
| GO:0033883 |
pyridoxal phosphatase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0046872 |
metal ion binding |
IDA: Inferred from Direct Assay |
F |
Data in Supplementary Table 2. With glucose-6-P as substrate, the Km for Mg2+ was 1 mM. |
complete | |||
| GO:0016791 |
phosphatase activity |
IDA: Inferred from Direct Assay |
F |
YigL had phosphatase activity on 12 of the 80 phosphorylated compounds tested. These were (in decreasing order of activity): pyridoxal-5-P, glucose-6P, 2-deoxy-glucose-6-P, erythrose-4-P, mannose-6-P, CMP, Fructose-6-P, dGMP, dIMP, glucosamine-6-P, ribose-5-P, and GMP. |
complete | |||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Source | Notes |
|---|---|---|---|---|
| edit table |
Notes
Structure and Physical Properties
Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Sequence |
MYQVVASDLD GTLLSPDHTL SPYAKETLKL LTARGINFVF ATGRHHVDVG QIRDNLEIKS YMITSNGARV HDLDGNLIFA HNLDRDIASD LFGVVNDNPD IITNVYRDDE WFMNRHRPEE MRFFKEAVFQ YALYEPGLLE PEGVSKVFFT CDSHEQLLPL EQAINARWGD RVNVSFSTLT CLEVMAGGVS KGHALEAVAK KLGYSLKDCI AFGDGMNDAE MLSMAGKGCI MGSAHQRLKD LHPELEVIGT NADDAVPHYL RKLYLS |
|---|---|
| Length |
266 |
| Mol. Wt |
29.707 kDa |
| pI |
4.4 (calculated) |
| Extinction coefficient |
24,410 - 24,910 (calc based on 9 Y, 2 W, and 4 C residues) |
| edit table |
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See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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Structure
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Structure figures
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| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
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| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
- ↑ 6.0 6.1 6.2 6.3 Kuznetsova E et al. (2006) Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family. J Biol Chem 281: 36149-61 PubMed EcoliWiki page
