ytfN:Gene - EcoliWiki
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ytfN:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

ytfN

Mnemonic
Synonyms

ECK4217, b4221, JW4180, ytfO[1]

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Notes

Location(s) and DNA Sequence

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StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

95.74 minutes 

MG1655: 4442135..4445914
gbrowse_img.php?coord=4441135..4446914&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 4448792..4452571
gbrowse_img.php?coord=4447792..4453571&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔytfN (Keio:JW4180)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC11010[3]

zjf-920::Tn10

Insertion at 4,442,823 bp in MG1655 (NC_000913)

adapted from Nichols et al.[4]

CAG12019 = CGSC7484[3]

Synonyms: zjh-901::Tn10, zjg-920::Tn10

ΔytfN770::kan

PMID:16738554[2]

CGSC:106086


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Notes

Genetic Resources

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ResourceResource TypeSourceNotes/Reference

JW4180

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAGTTTATGGAAAAAAATCAG

Primer 2:CCAAACTCGAACTGATAGAGCAA

cycA30::Tn10

Linked marker

CAG12073 = CGSC7482[3]

est. P1 cotransduction: 57% [4]

mdoB202::Tn10

Linked marker

CAG18430 = CGSC7488[3]

est. P1 cotransduction: % [4]

Synonyms:zjj-202::Tn10, zji-202::Tn10

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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G7875

EchoBASE (EcoliWiki Page)

EchoBASE:EB2407

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4221

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C5319

EcoGene (EcoliWiki Page)

EcoGene:EG12514

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YTFN

ASAP (EcoliWikiPage)

ASAP:ABE-0013811


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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  5. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page

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